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. 2016 Jul 24;7(33):52818–52831. doi: 10.18632/oncotarget.10810

Figure 3. DCs generated in the presence of minocycline are deficient in cytokine secretion.

Figure 3

Immature DCs generated from C57BL/6 mouse BM cells with (Mino-DCs) or without (Ctrl-DCs) minocycline were stimulated with 100 ng/ml LPS (A) or biodegradable OVA-microspheres (50 μg/ml as OVA) (B) for 24 h. For induction of IL-10 production, DCs were stimulated with 100 ng/ml LPS and 50 ng/mL IFN-γ. Cytokine secretion to culture supernatant was determined by ELISA. The data are presented as the mean ± SD of three separate experiments; * P < 0.05, **P < 0.01 compared with control.