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. 2016 Jul 6;7(33):52832–52848. doi: 10.18632/oncotarget.10451

Figure 4. Gelsolin forms a complex with Cu/ZnSOD.

Figure 4

(A-B) Proximity ligation assay (PLA) was performed in gelsolin-overexpressing and gelsolin-knockdown cells. PLA signals in red fluorescence were detected when cells were treated with both gelsolin and Cu/ZnSOD antibodies. Nuclei were counterstained with DAPI. Images were captured using Olympus DP72 microscope and cellSens software at 40X. (C) Endogenous Cu/ZnSOD was immunoprecipitated from lysates of C1 cells using Cu/ZnSOD antibody. Cu/ZnSOD and gelsolin were detected in the western blot using specific antibodies against Cu/ZnSOD and gelsolin as shown in lanes 2 and 4. The negative mouse IgG control in lanes 1 and 3 does not detect any protein band. Lane 5 shows the protein expression of gelsolin and Cu/ZnSOD in the C1 cell lysate. Data shown here is a representative of three independent experiments.