Figure 6. O₂^.- rescues invasion in gelsolin-depleted cells.

O₂^.- levels were induced in gelsolin-knockdown cells by silencing Cu/ZnSOD in wild-type HCT116 cells. (C) Western blot showing knockdown of gelsolin and Cu/ZnSOD in HCT116 cells. (B) Simultaneous knockdown of gelsolin and Cu/ZnSOD significantly increases O₂^.- levels in gelsolin-depleted cells. (C) Increasing O₂^.- levels by knockdown of Cu/ZnSOD rescues invasion in gelsolin-depleted HCT116 cells. Data shown are mean ± SD of at least three independent experiments. *p-value <0.05 versus controls using a two tailed Student's t-test. Values (mean ± SD ) are expressed as fold over the empty vector control or the control siRNA, which is arbitrarily set to one. The western blot gel picture is a representative image from three independent experiments.