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. 2004 Oct 21;32(19):e142. doi: 10.1093/nar/gnh139

Figure 2.

Figure 2

Cellular uptake and subcellular localization of AP-1 decoy ODNs in KB-3 cells. Representative micrographs of KB-3 cells transfected with fluorescent-labeled AP-1 decoy ODNs (represented as a green signal). (A) Panels (a–d) represent untransfected cells; panels (e–h) represent 12 h post-transfection. (a and e) Phase-contrast images; (b and f) green fluorescence images; (c and g) DAPI nuclear staining; (d and h) merged images. (B) Panels (a–c) represent untransfected cells; panels (d–f) represent 24 h post-transfection; panels (g–i) represent 48 h post-transfection. (a, d and g) Phase-contrast images; (b, e and h) are fluorescent images; (c, f and i) merged images. (C) Images were taken using the 100× objective 48 h post-transfection. (a) Phase-contrast; (b) green fluorescence; (c) merged images. Extranuclear fluorescence is evident.