Figure 1. High-throughput screening of BTIC-enriched mouse derived breast tumor cells identifies 5-HT antagonists as potential breast cancer therapeutics.

(A) Schematic depicting the experimental pipeline used to identify candidate compounds affecting tumor cell viability. (B) Scatter plot showing the screening results of the bioactive subset of the Canadian Compound Collection. (C) The histogram illustrates that the compound activities approximately conform to a Gaussian distribution. (D-G) The primary screening data illustrating the effect of the antagonists (fluoxetine, nortriptyline, paroxetine and sertraline) at a concentration of 5 μM in duplicate wells of 384-well plates. The positive control values represent the multiple tumor cell samples that were exposed to the vehicle.