Figure 5. Docetaxel and sertraline act synergistically to target tumorsphere-forming cells in vitro.

(A) Dose combination matrix for docetaxel and sertraline illustrating their effect on alamarBlue reduction. (B) Bar diagram demonstrating the synergistic effect of sertraline and docetaxel at the IC₅₀ for each drug in alamarBlue assays. The dashed line depicts the expected extent of inhibition of alamarBlue reduction if the effect of the drugs was additive. One-way ANOVA P < 0.001. (C) The effect of docetaxel (at two concentrations) and sertraline (at multiple concentrations) in combination on sphere formation.