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. 2016 Jul 16;7(33):53230–53244. doi: 10.18632/oncotarget.10637

Figure 7. S100P activates Ras/MEK/MAPK pathway.

Figure 7

(A) BT474 cells infected with shS100P lentivirus or control shGFP lentivirus were plated into soft agar to assess anchorage independent growth (AIG). Cells were grown in the presence of 10 μg/mL Trastuzumab for 3 weeks. (*P < 0.01, Student's t test). (B) Trastuzumab treatment inhibits p-MAPK levels in TzS BT474 cells, but the addition of recombinant S100P protein is sufficient to restore p-MAPK levels. (C) Knock down of S100P in TzR cells results in significant decrease in p-MAPK levels when cells are treated with trastuzumab, further supporting a role of S100P in trastuzumab-resistance.