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. 2016 Jul 22;7(33):53289–53298. doi: 10.18632/oncotarget.10795

Figure 1. PDIA6 promotes the proliferation of HeLa cells.

Figure 1

(A) HeLa cells were transfected with plasmid pCMV-sport6-PDIA6 or empty vector pCMV-sport6 and subjected to colony formation assay. (B) PDIA6 overexpression increased the colony number compared to negative control. *p < 0.05. (C) CCK-8 assay was employed to detect the effect of PDIA6 overexpression on the proliferation of HeLa cells. *p < 0.05. (D) The cell cycle of HeLa cells transfected with plasmid pCMV-sport6-PDIA6 or empty vector pCMV-sport6 was analyzed by flow cytometry. (E) Quantitive analysis of the cell cycle. *p < 0.05. (F) After transfection 48 h, cell lysates were immunoblotted with antibodies to Cyclin A1, Cyclin B1, Cyclin E1. (G) The quantitative analysis of protein was normalized to β-actin. *p < 0.05.