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. 2004 Nov 8;32(19):e155. doi: 10.1093/nar/gnh143

Figure 2.

Figure 2

PCR amplification of bisulfite-modified DNA. (A) Schematic representation showing the locations of oligonucleotides used for amplification. The positions of the oligonucleotides (designated A–E) are shown for both pAL300 and pAL301 relative to oligonucleotide ‘A’, with the arrowheads indicating the approximate extent of the palindromic sequences. Oligonucleotide sequences are shown in Table 1 as BiSuSK-‘X’ where ‘X’ refers to the corresponding oligonucleotide shown here (A–E). (B) Primary PCR amplification of bisulfite-modified pAL301 with the indicated oligonucleotides (i.e. a PCR amplification with oligonucleotides B and C is designated ‘BC’). (C) Hemi-nested PCR amplification of pAL300 PCR products AD and BE with the indicated primers. (M—100 bp ladder, Invitrogen.)