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. 2016 Jul 23;7(33):53350–53361. doi: 10.18632/oncotarget.10804

Figure 3. IL-17E-induced EGFR phosphorylation is dependent on Src and EGFR kinase activity.

Figure 3

IJG-1731, BT20, and MDA-MB468 cells were treated with the Src specific inhibitor AZM475271 (10 μM) (A), Iressa (0.25 μM) (B), or control DMSO and then stimulated with IL-17E (10 ng/ml), EGF (10 ng/ml) or with medium alone. EGFR and Src phosphorylation was then assessed by western blotting (left panel). Loading controls were determined by re-blotting the membranes with an anti-EGFR antibody. Data are representative of at least 2 independent experiments. In the right panel, densitometric quantification of Y416 Src and Y1086 EGFR, as shown in the representative blots, is expressed as the ratios of pY416 Src to EGFR and pY1086 EGFR to EGFR, as indicated.