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. 2016 Jul 23;7(33):53350–53361. doi: 10.18632/oncotarget.10804

Figure 5. IL-17E facilitates pEGFR and pSTAT3 co-translocation to the nucleus.

Figure 5

MDA-MB468 (A) or BT20 (B) cells were stimulated with IL-17E (10 ng/ml), EGF (10 ng/ml) or a combination of both. In the upper panel of (A) and (B), EGFR localization, as assessed by immunostaining with anti-EGFR antibodies (red). Nuclei were visualized with DAPI (blue). In the lower panel of (A) and (B), translocation of EGFR, STAT3α/β, and their phosphorylated counterparts from the cytoplasm to the nucleus, as assessed by western blotting using specific antibodies. Anti-β actin and H3 histone antibodies were used as loading controls for the cytoplasmic and nuclear fractions, respectively. Data are representative of 2 independent experiments. Densitometric quantifications are presented in Supplementary Figure 1 (MDA-MB468) and Supplementary Figure 2 (BT20).