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. 2016 Jul 23;7(33):53430–53442. doi: 10.18632/oncotarget.10802

Figure 3. Fractionated radiation-induced Notch ligands promote EMT in breast cancer cells.

Figure 3

(A) qRT PCR analysis for mRNA levels of Notch ligands in MCF7 breast cancer cell after fractionated irradiation. (B) Western blot and (C) immunocytochemistry for Notch ligands such as Jagged1 and DLL4 in MCF7 breast cancer cells after irradiation. (D) Protein expression for Notch2 activation in irradiated MCF7 cells that are transfected with siRNA targeting Jagged1 and DLL4. (E) Migration and invasion assays in transwells after irradiation of MCF7 that are transfected with siRNA targeting Jagged1 and DLL4. (F) Western blot and (G) immunocytochemistry for EMT markers after fractionated irradiation of MCF7 cells transfected with siRNA targeting Jagged1 and DLL4. β-actin was used as a loading control. Error bars represent mean ± S.D. of triplicate samples. *p < 0.05, **p < 0.01 and ***p < 0.001.