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. 2016 Jul 23;7(33):53430–53442. doi: 10.18632/oncotarget.10802

Figure 4. Fractionated radiation up-regulates Notch signaling through activation of STAT3.

Figure 4

(A) Western blot analysis for STAT3, NF-κB and Fra-1 phosphorylation status rafter irradiation of MCF7 breast cancer cells. (B) qRT-PCR for Notch2, Jagged-1 and DLL4 mRNA levels after irradiation of MCF7 cells that are transfected with siRNA targeting STAT3, NF-κB and Fra-1. (C) qRT-PCR for Notch2, Jagged1 and DLL4 mRNA levels after irradiation of MCF7 cells that are treated with STAT3 inhibitor (2 μM). (D) Western blot for NICD2, Jagged1 and DLL4 after fractionated irradiation (2 Gy × 3) of MCF7 that are treated with STAT3 inhibitor (2 μM). Error bars represent mean ± S.D. of triplicate samples. *p < 0.05, **p < 0.01 and ***p < 0.001.