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. 2016 Jul 23;7(33):53443–53458. doi: 10.18632/oncotarget.10803

Figure 2. miR-218 targets the MACC1-3′-UTR.

Figure 2

(A) Secondary structure prediction (RNAhybrid) of the binding between the seed sequence within the MACC1-3′-UTR (position 218 to 224 nt) and miR-218 (green-highlighted sequence) revealed a hybridization energy ΔG of –16.3 kcal/mol. Representation of MACC1-3′-UTR with miR-218 binding region and schematic presentation of mutated region of miR-218 seed sequence (red-labled bases were mutated). (B) Luciferase reporter assays of MACC1-3′-UTR wild type and mutated construct in HCT116, SW620 and SW480. Both wild type and mutated MACC1-3′-UTR are co-tranfected either with control-miR (ctrl) or miR-218. Percent luciferase activity was calculated with the respective control (*P < 0.05; **P < 0.01).