Figure 6. Methylation status of miR-218 host genes SLIT2 and SLIT3 promoters in CRC cells and CRC metachronous metastasis positive and negative specimens.
(A) miR-218 and SLIT2 expression were measured using qRT-PCR and positively correlated in a panel of CRC cell lines (R2 = 0.95). (B) Gel electrophoresis of PCR products of CRC cell lines obtained from methylation-specific PCR for miR-218_1 hosting gene SLIT2 and miR-218_2 hosting gene SLIT3. (C) miR-218 expression in metachronous metastasis positive and negative CRC tumor specimens in comparison to representative normal mucosa. RPII and RNUB6 served as internal controls. (D) Gel electrophoresis of PCR products of CRC tumor specimens obtained from methylation-specific PCR for miR-218_1 hosting gene SLIT2 and miR-218_2 hosting gene SLIT3.