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. 2016 Jul 23;7(33):53443–53458. doi: 10.18632/oncotarget.10803

Figure 7. 5-Aza treatment induced miR-218 expression and downregulated MACC1 gene expression.

Figure 7

SW480 and SW620 cells were treated with 5-Aza (2 μM) for 3 days and total RNA and protein were isolated. (A, B) qRT-PCR revealed induction of miR-218 and its host gene SLIT2 expression. (C) Luciferase activity of MACC1-3′-UTR was significantly reduced after 5-Aza (2 μM). (D) qRT-PCR and Western blot analysis revealed inhibition of MACC1 expression in the 5-Aza treated samples compared to DMSO treated controls. RNUB6, RPII and β-actin served as internal controls. Densitometric analysis of MACC1 protein bands normalized to β-actin represented as a mean value of triplicates, in comparison to the respective control.