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. 2016 Jul 13;7(33):53459–53470. doi: 10.18632/oncotarget.10557

Figure 3. Gelsolin inhibited EMT and actin cytoskeletal rearrangement to suppress the migration of gastric cancer cell.

Figure 3

A., Immunofluorescence staining for phalloidin (F-actin) in MGC cell with overexpression (mGSN) or knockdown (shGSN) of gelsolin. Scar bar, 10μm. White arrows showed the filamentous F-actin. Quantitative results of the actin fluorescence intensity were presented as mean ± SEM of triplicate experiments (*, P<0.05; **, P<0.01). B., Morphology of MGC cell stably transfected with shCtrl or shGSN was examined by phase contrast microscopy and immunofluorescence (RFP). C., Immunoblotting analyses of EMT-related proteins in MGC cell with overexpression or knockdown gelsolin. D., Luciferase activity assay of E-cadherin or N-cadherin promoter-driven luciferase expression in indicated cells after 48h post-transfection (*, P<0.05; **, P<0.01).