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. 2016 Jul 18;7(33):53526–53539. doi: 10.18632/oncotarget.10658

Figure 4. HRG-induced ErbB3 signaling impairs polarized morphogenesis of Caco-2 cells.

Figure 4

A. Caco-2 cells were grown in 3D cultures containing 2% FCS in the presence of the indicated growth factors (10 ng/ml) or in 2% FCS as a control (con). Three days post seeding cysts were lysed and analyzed by immunoblotting using the indicated antibodies (pErbB2(Tyr1248)). Tubulin was detected as a loading control. Shown is a representative blot (N=2). B. Caco-2 cells were transfected with non-targeting (siNT) and ErbB-receptor specific siRNAs, respectively. The next day, cells were seeded into 3D culture in the presence or absence of HRG (10 ng/ml). Three days post seeding lumen expansion was induced by CTX. The next day, cultures were fixed and stained with DAPI and phalloidin (not shown). The percentage of cysts with PSAL was determined (n>50; N=3).