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. 2016 Jul 18;7(33):53526–53539. doi: 10.18632/oncotarget.10658

Figure 5. HRG triggers hyperproliferation and disrupts the architecture of intestinal organoids.

Figure 5

A. Representative photographic series of intestinal crypts developing in the absence (con) or presence of 10 ng/ml EGF or HRG in 3D organoid culture. White arrowheads on day 3 indicate crypt domains. (Scale: 100 μm). B. Quantification of crypt domains per organoid on day three (N=3). C. Organoids were fixed on day 1 and stained with anti-E-cadherin antibody (green), phalloidin (F-actin; red) and DAPI (nuclei; blue) (scale: 50 μm). D. Capillary phospho-protein analysis of untreated organoids (con) and treated with EGF or HRG (N=2).