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. 2016 Jul 18;7(33):53571–53582. doi: 10.18632/oncotarget.10670

Figure 2. The high expression level of SLC12A1 in Hep 3B cell line.

Figure 2

A. SLC12A1 mRNA expression in one normal liver cell line (L02) and six HCC cell lines (Hep G2, QGY-7703, Hep 3B, SMMC-7721, HHCC, QGY-7701). B. The expression of SLC12A1 in one normal liver cell line (L0) and six hepatocellular carcinoma cell lines (HepG2, QGY-7703, Hep3B, SMMC-7721, HHCC, QGY-7701) detected by Western Blot. C. ChIP assay for H3K4me3, H3K9me3 and H3K27me3 at the promoter regions of SLC12A1. D. ChIP assay for acH3 and acH4 at the promoter regions of SLC12A1 by ChIP assay in L02, HepG2 and Hep3B cell lines. For C. and D., Real-time PCR of GAPDH on the immunoprecipitated DNA fractions was performed as additional internal controls. **, P < 0.01.