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. 2016 Jul 22;7(33):53797–53807. doi: 10.18632/oncotarget.10777

Figure 1. Memantine abrogates T-bet expression and proliferation of human T cells.

Figure 1

A. Human peripheral blood CD3+ T cells were stimulated with CD3 Abs (left), PMA/IO (middle) or irradiated, CD3-depleted PBMCs from another healthy donor in MLRs (right) +/- memantine. DNA synthesis was determined by 3[H]-Thymidine incorporation (cpm). Upper graphs represent a single experiment and lower graphs the mean relative proliferation + SD; n=4-6 and MLR n=3. B. T-bet expression in T cells reacting in MLRs +/- memantine was determined by flow cytometry. The histogram displays a representative experiment and data in the graph the mean + SD percentage of T-bet+ cells; n=5. C. The proliferation of naïve CD45RO- and memory CD45RO+ CD4+ human T cells was analyzed in MLRs +/- memantine. Graphs show the data of a representative experiment (left) and the mean relative proliferation + SD of 5 experiments (right). The significance of data was determined with Student's t test; p*<0.05, p**<0.01, and p***<0.001.