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. 2016 Jul 22;7(33):53837–53852. doi: 10.18632/oncotarget.10782

Figure 3. The results of western blotting and RT-PCR assay.

Figure 3

A. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1α, GLS1 and GLUD1 is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. B. shows the RT-PCR results of the mRNA expression of GLUD1 and GLS1, where the expression of the GLUD1 and GLS1 is significantly increased in the PDHA1 KO cells, with P values of 0.000 and 0.001, respectively, compared to the parental LNCaP cells. **P < 0.01, ***P<0.001.