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Nuclease studies showing circular structures. Lane 1, 36 nt precircle; lane 2, 42 nt precircle; lane 3, 48 nt precircle; lane 4, 54 nt precircle; lane 5, 60 nt precircle; lane 6, 36 nt nanocircle; lane 7, 42 nt nanocircle; lane 8, 48 nt nanocircle; lane 9, 54 nt nanocircle; and lane 10, 60 nt nanocircle. Partial digestions were carried out by incubation with S1 nuclease.
