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. Author manuscript; available in PMC: 2017 Feb 2.
Published in final edited form as: Leukemia. 2016 Jul 25;31(2):403–413. doi: 10.1038/leu.2016.201

Figure 4. Enhanced Repopulating Capacity of Slug−/− HSCs in Reconstituted Mice.

Figure 4

(A) Diagram of in vivo HSC repopulating assay. Donor Lin− BMCs from Slug+/+ or Slug−/− (CD45.2+) were infected with c-Kit-specific shRNA or control shRNA, and then injected together with helper splenocytes (CD45.1+) into recipient mice (CD45.1+) that received a prior irradiation (10 Gy). Ratio of donor-derived cells (CD45.2+) in peripheral blood was determined by flow cytometry.

(B) A representation of flow cytometric analysis of the ratio of donor-derived cells in peripheral blood was evaluated at 2 weeks after BM transplantation.

(C) Slug deficiency alleviated the impairment of in vivo HSC repopulating by knockdown of endogenous c-Kit. The percentage of donor-derived cells (CD45.2+) in peripheral blood was evaluated at 2 weeks (n = 4 mice) and 8 weeks (n = 3 mice) after BM transplantation, respectively. Data are representative of two independent experiments. All data are represented as mean ± SD. Two-tailed Student’s t tests were used to assess statistical significance (*P < 0.05, **p < 0.01, N.S., not significant, P > 0.05).

See also Figure S6, S7, and S8.