Table 1.
Alkaline Smith degradation of different synthetic oligosaccharides.
| Entry | Substrates | Productsa |
|---|---|---|
| 1 | GlcNAc-GlcA-(GlcNAc-GlcA)5-GlcNAc-GlcA-pNP | Monosaccharide-R |
| 2 | GlcNS-GlcA-(GlcNS-GlcA)5-GlcNS-GlcA-pNP | Monosaccharide-R |
| 3 | GlcNS6S-GlcA-(GlcNS6S-GlcA)5-GlcNS6S-GlcA-pNP | Monosaccharide-R |
| 4 | GlcNS-GlcA-GlcNS-IdoA-GlcNS-GlcA-pNP | Monosaccharide-R |
| 5 | GlcNS6S-GlcA-GlcNS6S3S-IdoA-GlcNS6S-GlcA-pNP | Monosaccharide-R |
| 6 | GlcNS6S-GlcA-(GlcNS6S-IdoA2S)5-GlcNS6S-GlcA-pNP | Undecassaccharide-Rb |
| 7 | GlcNS6S-GlcA-(GlcNS6S-IdoA2S)7-GlcNS6S-GlcA-pNP | Pentadecasaccharide-Rb |
| 8 | GlcNS-GlcA-(GlcNS-IdoA2S)7-GlcNS-GlcA-pNP | Pentadecassaccharide-Rb |
a
R=C4H3O3 (see 5 in Scheme 2 for structure of aglycone).
b
Confirmed by LC-MS (see Supplemental Information).