Fig. 5.

Downregulation of TMEM59L attenuates neuron apoptosis induced by hydrogen peroxide insult. a Schematic illustration of the AAV2/8 shRNA construct targeting mouse Tmem59l. b Primary cortical neurons were infected with AAV-59l-shRNA or AAV-sc-shRNA for 7 days at day 3 in vitro (DIV3). Total RNAs were extracted, reverse transcribed, and subjected to qRT-PCR. The mRNA levels of Tmem59l were normalized to those of β-actin and compared to that of control (set as one arbitrary unit). n = 3, ***p < 0.001. c, d Primary cortical neurons were infected with AAV-59l-shRNA or AAV-sc-shRNA for 7 days at DIV3 and then treated with 300 µM H₂O₂ or not (control) for another 5 h. c Indicated proteins from neuronal lysates were analyzed by Western blot and d quantified by densitometry for comparison. n = 3; ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001