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. 2017 Jan 30;19(3):185–195. doi: 10.1016/j.neo.2016.12.010

Figure 1.

Figure 1

(A) qRT-PCR analysis of EPSIN 3 in HCT116 p53−/− and p53+/+ cells. After incubation with medium containing 2 μg/ml of ADR for 2 h, HCT116 p53−/− and p53+/+ cells were incubated with culture medium that did not contain ADR for the indicated hours. (B - D) A549, U2OS and HepG2 cells were transfected with sip53 24 h before treatment with ADR. siEGFP and no transfection (noTF) were used as controls. At 48 h after treatment with 2 μg/ml of ADR, total RNA was subjected to qRT-PCR. (E, F) qRT-PCR analysis of EPSIN 3 in U373 MG (p53 mutant) and H1299 (p53 null) cells infected with adenovirus expressing either p53 (Ad-p53) or LacZ (Ad-LacZ) at a multipli2city of infection (MOI) of 10 or 20. (F) qRT-PCR analysis of Epsin 3 expression in MEF cells obtained from p53−/− and p53+/+ mice. The cells were harvested at the indicated times after 2 μg/ml ADR treatment for 2 h. Error bars represent the S.D. (n = 3). Gapdh was used to normalize the expression levels. (H) qRT-PCR analysis of Epsin 3 expression in the thymus of X-ray-irradiated p53−/− and p53+/+ mice (10 Gy) (n = 3 per group). Mice were sacrificed 24 h after irradiation with 10 Gy of X-rays. β-actin was used to normalize the expression levels in all qRT-PCR analyses unless otherwise noted.