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. 2017 Jan 30;19(3):185–195. doi: 10.1016/j.neo.2016.12.010

Figure 4.

Figure 4

(A) H1299, HCT116 and U373 MG cells transfected with either an EPSIN 3-expressing plasmid or mock vector were cultured in medium containing 0.8, 0.5 and 0.5 mg/ml of geneticin, respectively, for 2–3 weeks. Error bars represent the S.D. (n = 3). The P value was calculated by using Student's t tests. (B) At 24 h after transfection of each siRNA, HCT116 cells were seeded and cultured on ultra-low-attachment plates. Then, 24 h after plating, HCT116 cells were treated with 1 μg/ml ADR for 48 h and subjected to cell proliferation analysis. Relative cell viability was calculated by dividing the absorbance of ADR-treated cells by that of untreated cells. Error bars represent the S.D. (n = 3). (C) Western blot analysis was performed using HCT116 cells treated as described above. siRNA against EGFP was used as a control.