Table 2.
Test results and sensitivity of HT-J qPCR under different test conditions and interpretation criteria assuming fecal culture (FC) as the gold standard.
| Test conditions and interpretation criteria | HT-J qPCR test resultsa |
Sensitivity (%) (95% confidence interval) | ||||
|---|---|---|---|---|---|---|
| HT-J positive and FC positive | HT-J positive and FC negative | HT-J negative and FC negative | HT-J negative and FC positive | Total | ||
| (1) Undiluted DNA extract | 44 | 13 | 203 | 36 | 296 | 55.0 (43.47, 66.15) |
| (2) Diluted DNA extract (after accounting for dilution)b | 61 | 38 | 178 | 19 | 296 | 76.25 (65.42, 85.05) |
| (3) Result of 1 and 2 used in parallel | 64 | 43 | 173 | 16 | 296 | 80.0 (69.56, 88.11) |
aAt cut off ≥0.001 pg DNA/qPCR reaction for positive test result.
bDNA quantified from HT-J qPCR of five-fold diluted DNA extract was multiplied by five to account for dilution of the DNA extract and the new value was used to interpret the test results.