FIGURE 7.

Cellulose degradation products in the medium and in the cells. (A,B) The cellulose degradation products of the wild type strain (A) and ΔbglA/bglB (B) in the medium. Cells were cultured in PY6 glucose medium to middle exponential phase and resuspended to a concentration of 100 μg of protein per milliliter in Stanier medium supplemented with 0.4% (wt/vol) cellulose. (C) The intracellular cello-oligosaccharides of the wild type strain under different culture conditions. Line 1, control sample, the residual metabolites in the Stanier medium after washing the cells for the third time; line 2, cells in glucose culture; line 3, free cells in cellulose culture; line 4, cellulose binding cells in cellulose culture. (D) The intracellular cello-oligosaccharides of cellulose binding cells in cellulose culture. All the samples were extracted from the same amount (100 μg of protein per milliliter) of the wild type strain and β-glucosidase deletion mutant.