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. 2017 Feb 2;8:151. doi: 10.3389/fmicb.2017.00151

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Copyright © 2017 Zhang, Chen, Li, He, Chen and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of intracellular ROS in Cryptococcus laurentii following treatment with and without exogenous H₂O₂. (A) Microscopic images of C. laurentii cells stained with 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). (B) Percentage of C. laurentii cells exhibiting visible ROS accumulation. (C) Statistical analysis of the fluorescence intensity of C. laurentii cells. Vertical bars represent standard errors of the mean of three independent experiments. Columns with different letters indicate significant differences (P < 0.05).