Skip to main content
. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Dev Biol. 2016 Jun 20;416(2):324–337. doi: 10.1016/j.ydbio.2016.06.025

Figure 1.

Figure 1

Timelapse confocal microscopy reveals severe defects in optic cup formation in lama1UW1 mutant.

(A–H) Single confocal slices from 4D datasets of optic vesicle morphogenesis, 12.5–24.5 hpf. EGFP-CAAX (membranes, green), H2A.F/Z-mCherry (nuclei, magenta).

(A–D) Optic cup formation in control embryo. (E–H) Optic cup formation in lama1UW1 mutant embryo. Dorsal views; scale bar, 50 µm.

(I–M) Localization of laminin protein. (I–L) In control embryos, laminin protein is found lining basal surfaces of developing eye and brain. (M) lama1UW1 mutant embryo reveals absence of laminin protein from early stages of optic vesicle development.

nk, neural keel; ov, optic vesicle; ec, ectoderm; br, brain; RPE, retinal pigmented epithelium; nr, neural retina; le, lens. A, anterior; P, posterior; M, medial; L, lateral.