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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Dev Biol. 2016 Jun 20;416(2):324–337. doi: 10.1016/j.ydbio.2016.06.025

Figure 4.

Figure 4

Apoptosis is increased in lama1UW1 mutant embryos but is not the underlying cause of morphogenesis defects.

(A-A”) Control embryos show little apoptotic cell death. (B-B”) lama1UW1 mutant embryos contain a significant number of dying cells. (C-C”) Injection of Bcl-xL RNA (100 pg) rescues apoptosis in lama1UW1 mutant embryos, however optic cup morphogenesis defects are still apparent.

(A,B,C) Antibody staining for activated caspase-3. (A’,B’,C’) TOPRO-3 counterstain for nuclei. (A”, B”, C”) Merged images. dashed blue line, boundary of optic cup. Dorsal views; scale bar, 50 µm. br, brain; nr, neural retina; le, lens. A, anterior; P, posterior; M, medial; L, lateral.