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Enzyme treatment affects in situ muscle activation properties. Airflow modulation (mean percentage ±1 SD) by the ventral tracheobronchial muscle during increasing stimulation frequency is shown relative to that at 10-Hz stimulation (100%). Heparitinase-treated muscle shows significantly reduced modulation depth at lower stimulation frequencies than vehicle-treated controls (n = 4 for each condition; **P < 0.005, *P < 0.05).
