Fig. 5.

Localization of fructokinase (KHK) mRNA by in situ hybridization (ISH) in mouse brain. A: Nissl stained coronal section (http://brainmaps.org/) showing the relative location of B and C in the whole brain. B and C: ISH staining for khk expression (dark color) was shown in the hypothalamic regions of a coronal section of mouse brain that include the supraoptic nucleus (SON), the paraventricular nucleus (PVN) and the suprachiasmatic nucleus (SCN) (×4 magnification). Cells neighboring the PVN, SON and SCN were negative. D–F: higher magnification (×20) pictures showed positive ISH signals in a subset of cells of the PVN (D), SON (E) and SCN (F). Positive signals are shown by the white arrowheads. Scale bars in B and C = 150 μm; scale bars in all other panels = 50 μm. OC, optic chiasm; IIIV, third ventricle.