Figure 5.

The percentage of Th1 cells is increased in Gnaq−/− BM chimeras suffering from inflammatory arthritis. (a) Single cell suspension was deprived from spleen of Gnaq−/− BM chimeras suffering from inflammatory arthritis and WT controls, stimulated with PMA, ionomycin, and monensin for 4 hours. After culture, cells were stained with PE-conjugated anti-CD4, followed by intracellular staining with FITC-conjugated anti-IFN-γ, and analyzed by flow cytometry. Gated on CD4⁺ cells. (b) The percentage of Th1 cells is presented as mean ± SE, ^∗∗P < 0.05, n = 3. The result is representative of three independent experiments.