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. 2017 Jan 19;2017:6487825. doi: 10.1155/2017/6487825

Figure 1.

Figure 1

Effect of long-term treatment of nLDL on the proliferation of HUVECs. Young HUVECs (PDL, 12~15) were subcultured at every third day of each subculture with media exchange (a) and cultured continuously in the same culture dish with media exchange (b), for up to 9 days. The cells were treated with various concentrations of nLDL (0, 2, 5, and 10 μg protein/mL) concomitantly with media exchange every 3 days at both culture systems (1st, 2nd, and 3rd Tx). Cellular proliferation of the cells was analyzed by tetrazolium salt method. The degree of cellular proliferation was expressed as the relative ratio of cell number. The dose- and time-dependent differences in cellular proliferation between groups were analyzed statistically by repeated measures ANOVA assay (p < 0.01). Each nLDL-treated group was also compared with the respective nLDL-untreated group by independent t-test. ∗p < 0.05; ∗∗p < 0.01. Each result represents the mean ± SD (n = 6).