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. 2017 Jan 19;2017:6487825. doi: 10.1155/2017/6487825

Figure 2.

Figure 2

Senescence induction in HUVECs by long-term treatment of nLDL (0, 2, 5, and 10 μg protein/mL), for up to 9 days. (a) Quantitative assay of SA-β-Gal activity after nLDL treatment; (b), SA-β-Gal activity staining after nLDL treatment. HUVECs were treated with nLDL at the start of each subculture and assayed for SA-β-Gal activity at the end of each subculture. The SA-β-Gal activity was expressed as the generation rate of 4-methylumbelliferone (MU)/μg protein against that of the nLDL-untreated group. The dose- and time-dependent differences in SA-β-Gal activity between groups (a) were analyzed statistically by repeated measures ANOVA assay (p < 0.01). Each nLDL-treated group was also compared with the respective nLDL-untreated group by independent t-test (∗p < 0.01). Each result represents the mean ± SD (n = 3).