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. 2017 Jan 19;2017:6487825. doi: 10.1155/2017/6487825

Figure 4.

Figure 4

Effect of LDL receptor (LDLR) blocking with antibody on the nLDL-induction of senescence in HUVECs. The cells were pretreated with anti-LDLR antibody (20 μg protein/mL) before nLDL (10 μg protein/mL) treatment. The cells were cultured for up to 6 days. Senescence induction was carried out by SA-β-Gal activity assay in subcultured cells (a) and cellular proliferation by tetrazolium salt staining in continuously cultured cells (b), every 3 days. The time-dependent difference in SA-β-Gal activity or cellular proliferation between treatment groups was analyzed statistically by repeated measures ANOVA assay (p < 0.01). And also, each nLDL-treated group was compared with the respective nLDL-untreated group (∗p < 0.01) and each anti-LDLR antibody plus nLDL-treated group was compared with the respective nLDL-treated group (†p < 0.01) by independent t-test. Each result represents the mean ± SD (n = 6).