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Ectopic expression of HATgPRELID. (A) Western blot assay using anti-HA antibody against HATgPRELID-expressing parasites shows a band at the predicted molecular mass of 55 kDa that is absent in parental RHΔHX parasites. TgAldolase was probed as a loading control. (B) IFA of the HATgPRELID expressed in tachyzoites shows colocalization with the mitochondrial protein F1B-ATPase. Bar, 2 µm.
