FIG 3.

δCURE-Epo does not induce polycythemia in transgenic mice. (A) Mating strategy to obtain δCURE-Epo::Epo^+/− mice. (B) Copy numbers of the δCURE-Epo transgene in each δCURE-Epo::Epo^+/− mouse line. The structures of the δCURE-Epo transgene and primer sets for copy number detection (blue arrows) are depicted above the qPCR results. The qPCR results were standardized utilizing the Gata2 or Myb locus. Estimated transgene copy numbers are depicted in blue. The data are means and SD (n = 3 to 8). (C) Hematocrit percentages for each line of adult male δCURE-Epo::Epo^+/− mice. Note that no polycythemia was observed in these transgenic mice. The data are means and SD (n = 3 to 6).