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. 2017 Feb 2;7:41519. doi: 10.1038/srep41519

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Copyright © 2017, The Author(s)

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(a) 10 μg, 3 μg and 1 μg of a total membrane fraction from Xenopus egg extracts were separated on a 15% SDS-PAGE and analyzed by western blotting using antisera against full-length SCL1/BC08 (aa 1–97, antiserum A and B) in a 1:10,000 dilution and antisera against the nucleoplasmic domain (aa 1–76, antiserum C and D) in a 1:1,000 dilution. Molecular size markers as well as the position of SCL1/BC08 are indicated. (b) SCL1/BC08 was immunprecipitated from a solubilized total membrane fraction from Xenopus egg extracts using antisera against full-length SCL1/BC08 (antiserum A and B) and antisera against the nucleoplasmic domain (antiserum C and D). Antibody bound proteins were eluated with SDS sample buffer and separated together with 30% and 100% of the corresponding starting material on a 15% SDS-PAGE. After western blotting samples were analyzed with α- SCL1/BC08 antiserum A.