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. 2017 Feb 2;7:41519. doi: 10.1038/srep41519

Figure 6. Antisera raised against MISTIC-fusion proteins can be efficiently affinity purified.

Figure 6

An antiserum raised against a MISTIC-fusion with the full-length transmembrane nucleoporin POM121 was affinity purified using the MISTIC-POM121 protein coupled to Affigel 10 matrix as bait. The unpurified antiserum (at a 1:1,000 dilution) as well as the affinity purified antibodies (at a 0.1 mg/ml and 0.03 mg/ml dilution) were tested by western blotting after 6% SDS-PAGE of 10 μg, 3 μg and 1 μg of a total membrane fraction from Xenopus egg extracts. Molecular size markers as well as the position of POM121 are indicated.