Figure 7. Antibodies raised against full-length POM121 and NDC1 inhibit nuclear reformation.

(a) Xenopus egg extract membranes were preincubated with control or α-POM121, α-NDC1 or α-GP210 IgG, respectively, and used in nuclear assembly reactions. Membranes were stained with DiIC18 (red) and chromatin with DAPI (blue) and samples analyzed by confocal microscopy. As previously reported for antibodies against soluble domains of POM121 and NDC1²²,³⁰, also antibodies against full-length POM121 and NDC1 but not GP210 block formation of a closed nuclear envelope around sperm chromatin. (b) 100 randomly chosen chromatin substrates per reaction from samples from (a) were analyzed for closed nuclear envelope formation. The average of three independent experiments are shown, individual data points are indicated.