Table 2. List of in vitro experiments comparing platelet storage at 4°C versus room temperature.

Authors	Journal	Year	4°C versus room temperature
Johnson et al. 18	Transfusion	2016	Reduction of glycolysis Increased expression of P-selectin Faster thrombin generation Faster clot formation, equal strength
Bynum et al. 22	Transfusion	2016	Less oxidative stress Stronger clot Increased response to aggregating agents Better aggregation in shear stress conditions
Getz et al. 6	Transfusion	2016	No difference in platelet content in the first 5 days of storage No difference in rotation thromboelastometry (ROTEM) pattern after 5 days of storage
Wood et al. 10	Transfusion	2016	Decreased expression of GPIB, GPIX, GPIIB, and GPIV (easier von Willebrand factor attack) Increased expression of P-selectin, tetraspanin, and phosphatidylserine Cytoskeleton protein modifications corresponding to activation state (proteomics) Increased expression of CD62P, CD63, and annexin V
Baimukanova et al. 15	Transfusion	2016	Increased aggregation potential
Reddoch et al. 9, 17	Shock Shock	2014 2016	Increased expression of CD40 and P-selectin Increase of intracellular free calcium Increase of dense granule release of ATP Accelerated thrombin generation More pronounced response to ADP, collagen, and TRAP (thrombin receptor-activating peptide) Faster, stronger, and more durable clot
Mondoro and Vostal 23	Platelet	2002	Increased response to ADP and epinephrine Stronger clot resistance to disaggregating agents No spontaneous aggregation
Connor et al. 12	Transfusion	1996	Reduced expression of GMP-140 ADP response of 250%; collagen response of 100% at more than room temperature
Triulzi et al. 11	Transfusion	1992	Increased expression of GMP-140
Rinder et al. 7	Transfusion	1990	Increased expression of GMP-140
Becker et al. 16	Transfusion	1983	More pronounced ADP response

The main results about platelet marker expression and platelet functionality are reported.