Table 3.
Summary of mass spectrometry data.
| Phage | ORF | Putative function | No. of peptides | Coverage (%) |
|---|---|---|---|---|
| 93 (FIGURE 2A. CONTROL) | ||||
| 07 | Portal protein | 12 | 38 | |
| 15 | Major tail protein | 10 | 51 | |
| 16 | Tail protein extension | 6 | 53 | |
| 19 | TMP | 19 | 24 | |
| 20 | Dit | 14 | 33 | |
| 21 | Tal | 8 | 24 | |
| 23 | RBP | 11 | 58 | |
| 93 PVP (FIGURE 2A 3% PVP) | ||||
| Band 1: | 16 | Tail protein extension | 4 | 44 |
| 15 | Major tail protein | 3 | 12 | |
| Band 2: | 15 | Major tail protein | 4 | 20 |
| Band 3: | 16 | Tail protein extension | 2 | 17 |
| 16 | Major tail protein | 4 | 29 | |
| 7 | Portal protein | 3 | 10 | |
| 8 | Protease | 6 | 48 | |
| 10 | Hypothetical structural protein 1 | 3 | 44 | |
| 11 | Hypothetical structural protein 2 | 2 | 27 | |
| 13 | Hypothetical structural protein 3 | 1 | 14 | |
| 14 | Hypothetical structural protein 4 | 4 | 35 | |
Structural proteins extracted from purified phage 93 particles before (93) and after (93 PVP) exposure to 3% w/v PVP via electrospray ionization-tandem mass spectrometry (ESI-MSI/MS). A minimum of either two independent unique peptides or 5% sequence coverage were used as threshold values.