Table 1. Primer sequences for qRT-PCR.
Gene | Primer sequence (5′-3′) | Product size |
---|---|---|
OCN | F: GAATCCCGCAAAGGTG | 185 bp |
R: GCTGACCACATTGGCTT | ||
VEGF | F: AAGTCCTGGAGCGTTCCCTGT | 168 bp |
R: GACAAGCCCAGGCGGTGA | ||
GAPDH | F: AAGGTCGGAGTCAACGGATTT | 372 bp |
R: GGCATCAGCAGAAGGAGCAG |
Note: OCN, osteocalcin; VEGF, vascular endothelial growth factor, qRT-PCR, quantitative real-time polymerase chain reaction.