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. 2017 Feb 2;7:41351. doi: 10.1038/srep41351

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Splenic CD8⁺ T cells from Eβ ^+/+:E8I-Cre and Eβ ^f/f:E8I-Cre mice were stimulated with immobilized anti-CD3 and soluble anti-CD28 antibodies. Two days after stimulation, cells were harvested and were kept in culture with medium supplemented with 20 units/ml of mIL-2. TCRβ expression kinetics after stimulation are shown as histograms. (B) Dot plots showing CD25 and CD69 activation markers, and TCRb expression and CFSE as a marker for cell proliferation at the indicated days. (C) ChIP assay measuring H3K4 and K27 tri-methylation levels at indicated regions in the Tcrb locus in TCRβ⁻ and TCRβ⁺ cells, which were prepared at 12 days after TCR stimulation. Combined data from three independent ChIP experiments is shown.