Figure 3. Less efficient C- and T-tailing by MMLV-RT of DNA substrates with A or T at the 3′ end that is alleviated by dGMP and dAMP.

(a) Tailing efficiencies on different DNA ends. Five different DNA molecules at 10 nM were subjected to A-, C-, G- or T-tailing for five min, and the size distribution of the reaction products were analyzed. (b) dNMPs enhanced less efficient tailing. FAM69A and FAM69T DNAs were subjected to C- and T-tailing reactions in the presence of dGMP and dAMP, respectively. Results are shown as averages of triplicate experiments.