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. 2017 Feb 2;7:41608. doi: 10.1038/srep41608

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Copyright © 2017, The Author(s)

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(A) qPCR for the Palmd, Myogenin, MyoD and MyHC expression profile during C2C12 myoblast differentiation at the indicated time points, showed up-regulation of Palmd after differentiation. Data are presented as mean ± s.e.m., n = 3 per group. (B) The protein levels of Palmd, Myogenin, MyHC during C2C12 myoblast differentiation showed a similar result. GM: growth medium, DM: differentiation medium. (C) Immunofluorescence staining for Palmd (green) together with Phalloidin (red) and nucleus (blue) staining in myoblast and myotube. Scale bar = 25 μm.